Total Body Weight (TBW) vs Lean Body Mass (LBM)Physiological Mass Metric
Fat-free tissue mass representing skeletal muscle, bone, organs, and intracellular water. For individuals with body fat exceeding 25% in males or 32% in females, calculating protein needs on Total Body Weight causes massive over-prescription; clinical sports nutrition indexes targets to LBM (2.2-2.6 g/kg LBM) to prevent unnecessary metabolic renal load.
Default: Auto-calculated via Boer formula or user body fat %
Myofibrillar Protein Synthesis (MPS) Leucine TriggerBiochemical Threshold
The minimum essential amino acid dose required to activate the intracellular mTORC1 phosphorylation pathway. Achieved when a single meal delivers approximately 2.7 to 3.5 grams of L-leucine, equivalent to 0.40 g/kg of high-biological-value protein (or roughly 30 to 45 grams total protein per feeding).
Default: 0.40 g/kg per feeding opportunity
Age-Related Anabolic Resistance Factor (Age 65+)Clinical Adjustment Factor
Age-mediated attenuation of splanchnic extraction, muscle microvascular perfusion, and postprandial mTOR signaling. While younger adults trigger maximal MPS with 0.24 g/kg per feeding, adults over 65 require 0.40 to 0.45 g/kg per meal (1.2 to 1.6 g/kg/day total) to overcome the anabolic threshold and prevent sarcopenic frailty.
Default: 1.25x per-meal threshold scaling for age >= 65
Hypocaloric Protein Sparing MultiplierMetabolic Multiplier
In a negative energy balance (caloric deficit), hepatic gluconeogenesis upregulates amino acid oxidation for glucose synthesis. To spare lean skeletal muscle from catabolism, recommended intake increases from 1.6-1.8 g/kg to 2.2-2.6 g/kg LBM (or Helms et al. 2.3-3.1 g/kg FFM) inversely proportional to deficit depth.
Default: 2.20 g/kg LBM under moderate caloric deficit
Digestible Indispensable Amino Acid Score (DIAAS)Bioavailability Correction
The standardized ileal amino acid digestibility standard adopted by the FAO. Isolated plant protein sources (legumes, grains) have lower DIAAS scores (0.60-0.85) compared to animal proteins (whey, milk, eggs, beef at 1.15-1.30), requiring a 10% to 15% increase in total daily gross plant protein to match essential amino acid delivery.
Default: +12% total intake adjustment for plant-based diets
Thermic Effect of Food (TEF) & Caloric DensityBioenergetic Constant
The energetic cost of digesting, absorbing, and metabolizing dietary macronutrients. Protein possesses an obligatory thermic cost of 20% to 30% of its caloric value (compared to 5-10% for carbohydrates and 0-3% for fats). Consequently, 100 kcal of consumed protein yields a net physiological energy value of only 70-80 kcal.
Default: 4.0 gross kcal per gram (25% mean TEF)